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dc.contributor.authorBélanger, Mireille
dc.contributor.authorDesjardins, Paul
dc.contributor.authorChatauret, Nicolas
dc.contributor.authorRose, Christopher
dc.contributor.authorButterworth, Roger F.
dc.date.accessioned2013-04-28T20:29:50Z
dc.date.available2013-04-28T20:29:50Z
dc.date.issued2005-05
dc.identifier.urihttp://hdl.handle.net/1866/9594
dc.description.sponsorshipCIHRen
dc.subjectAcute liver failureen
dc.subjectAstrocytesen
dc.subjectBrain edemaen
dc.subjectDéfaillance hépatique aigüeen
dc.subjectEncéphalopathie hépatiqueen
dc.subjectHepatic encephalopathyen
dc.subjectOedème cérébralen
dc.subjectPeripheral-type benzodiazepine receptoren
dc.subjectPregnenoloneen
dc.subjectPrégnénoloneen
dc.subjectTSPO protein, humanen
dc.titleMild hypothermia prevents brain edema and attenuates up-regulation of the astrocytic benzodiazepine receptor in experimental acute liver failure
dc.typeArticleen
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine. Centre de recherche du CHUMfr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecinefr
dc.identifier.doi10.1016/j.jhep.2004.12.029
dcterms.abstractBACKGROUND/AIMS: Mild hypothermia has proven useful in the clinical management of patients with acute liver failure. Acute liver failure in experimental animals results in alterations in the expression of genes coding for astrocytic proteins including the "peripheral-type" (astrocytic) benzodiazepine receptor (PTBR), a mitochondrial complex associated with neurosteroid synthesis. To gain further insight into the mechanisms whereby hypothermia attenuates the neurological complications of acute liver failure, we investigated PTBR expression in the brains of hepatic devascularized rats under normothermic (37 degrees C) and hypothermic (35 degrees C) conditions. METHODS: PTBR mRNA was measured using semi-quantitative RT-PCR in cerebral cortical extracts and densities of PTBR sites were measured by quantitative receptor autoradiagraphy. Brain pregnenolone content was measured by radioimmunoassay. RESULTS: At coma stages of encephalopathy, animals with acute liver failure manifested a significant increase of PTBR mRNA levels. Brain pregnenolone content and [(3)H]PK 11195 binding site densities were concomitantly increased. Mild hypothermia prevented brain edema and significantly attenuated the increased receptor expression and pregnenolone content. CONCLUSIONS: These findings suggest that an attenuation of PTBR up-regulation resulting in the prevention of increased brain neurosteroid content represents one of the mechanisms by which mild hypothermia exerts its protective effects in ALF.en
dcterms.languageengen
UdeM.VersionRioxxVersion acceptée / Accepted Manuscript
oaire.citationTitleJournal of hepatology
oaire.citationVolume42
oaire.citationIssue5
oaire.citationStartPage694
oaire.citationEndPage699


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