☃ ❄ ⛄ ☃ ❄ ⛄ CONGÉ DES FÊTES 2018 : Veuillez noter qu'il n'y aura pas de suivi des dépôts des thèses, mémoires et travaux étudiants après le 20 décembre 2018. Retour aux délais réguliers dès le 3 janvier 2019.
Increased expression of "peripheral-type" benzodiazepine receptors in human temporal lobe epilepsy: implications for PET imaging of hippocampal sclerosis
Article [Accepted Manuscript]
Is part ofMetabolic Brain Disease ; 17(1)
- Université de Montréal. Faculté de médecine
- Université de Montréal. Faculté de médecine. Centre de recherche du CHUM
Increased binding sites for "peripheral-type" benzodiazepine receptor (PTBR) ligands have been described in a wide range of neurological disorders including both human and experimental epilepsy. This study was undertaken to assess PTBR expression in relation to the presence of hippocampal sclerosis in human temporal lobe epilepsy (TLE). For this purpose, hippocampal CA1 subfields were dissected from surgical samples from patients with therapy-refractive TLE with (n = 5) or without (n = 2) hippocampal sclerosis and from age-matched nonepileptic postmortem controls (n = 5). PTBR expression was assessed by immunohistochemistry and reverse-transcription polymerase chain reaction. Receptor sites were evaluated using an in vitro binding assay and the selective PTBR ligand [3H]PK11195. Epileptic patients with hippocampal sclerosis showed increases in PTBR binding sites, immunoreactivity, and mRNA expression compared to both nonsclerotic TLE patients and postmortem nonepileptic controls. Induction of PTBR expression and binding sites were directly correlated with the presence of hippocampal sclerosis and the accompanying reactive gliosis.
Sauvageau, A., Desjardins, P., Lozeva, V., Rose, C., Hazell, A. S., Bouthillier, A. and Butterworth, R. F. (2002) Increased expression of "peripheral-type" benzodiazepine receptors in human temporal lobe epilepsy: implications for PET imaging of hippocampal sclerosis. Metab Brain Dis 17(1):3-11