Association between disruption of CD4 receptor dimerization and increased human immunodeficiency virus type 1 entry
dc.contributor.author | Bourgeois, Rachel | |
dc.contributor.author | Mercier, Johanne | |
dc.contributor.author | Paquette-Brooks, Isabelle | |
dc.contributor.author | Cohen, Éric A. | |
dc.date.accessioned | 2007-01-05T21:56:43Z | |
dc.date.available | 2007-01-05T21:56:43Z | |
dc.date.issued | 2006 | |
dc.identifier.uri | http://www.retrovirology.com/content/3/1/31 | |
dc.identifier.uri | http://hdl.handle.net/1866/662 | |
dc.format.extent | 397203 bytes | |
dc.format.mimetype | application/pdf | |
dc.rights | Ceci est un article en accès libre diffusé sous une licence Creative Commons Paternité laquelle permet une libre utilisation, diffusion et reproduction de l'article sous toutes formes, à la condition de l'attribuer à l'auteur en citant son nom. This is an open access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 | |
dc.title | Association between disruption of CD4 receptor dimerization and increased human immunodeficiency virus type 1 entry | |
dc.type | Article | |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine. Département de microbiologie, infectiologie et immunologie | fr |
dc.identifier.doi | 10.1186/1742-4690-3-31 | |
dcterms.abstract | BACKGROUND:Human immunodeficiency virus (HIV) enters target cells by a membrane fusion process that involves a series of sequential interactions between its envelope glycoproteins, the CD4 receptor and CXCR4/CCR5 coreceptors. CD4 molecules are expressed at the cell surface of lymphocytes and monocytes mainly as monomers, but basal levels of CD4 dimers are also present at the cell surface of these cells. Previous evidence indicates that the membrane distal and proximal extracellular domains of CD4, respectively D1 and D4, are involved in receptor dimerization.RESULTS:Here, we have used A201 cell lines expressing two CD4 mutants, CD4-E91K, E92K (D1 mutant) and CD4-Q344E (D4 mutant), harboring dimerization defects to analyze the role of CD4 dimerization in HIV-1 entry. Using entry assays based on ß-lactamase-Vpr or luciferase reporter activities, as well as virus encoding envelope glycoproteins derived from primary or laboratory-adapted strains, we obtained evidence suggesting an association between disruption of CD4 dimerization and increased viral entry efficiency.CONCLUSION:Taken together, our results suggest that monomeric forms of CD4 are preferentially used by HIV-1 to gain entry into target cells, thus implying that the dimer/monomer ratio at the cell surface of HIV-1 target cells may modulate the efficiency of HIV-1 entry. | en |
dcterms.description | Affiliation: Département de microbiologie et immunologie, Faculté de médecine, Université de Montréal & Institut de Recherches Cliniques de Montréal | |
dcterms.isPartOf | urn:ISSN:1742-4690 | |
UdeM.VersionRioxx | Version acceptée / Accepted Manuscript | |
oaire.citationTitle | Retrovirology | |
oaire.citationVolume | 3 |
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Usage rights : Ceci est un article en accès libre diffusé sous une licence Creative Commons Paternité laquelle permet une libre utilisation, diffusion et reproduction de l'article sous toutes formes, à la condition de l'attribuer à l'auteur en citant son nom. This is an open access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.