Estrogen and testosterone in concert with EFNB3 regulate vascular smooth muscle cell contractility and blood pressure
dc.contributor.author | Wang, Yujia | |
dc.contributor.author | Wu, Zenghui | |
dc.contributor.author | Thorin, Eric | |
dc.contributor.author | Tremblay, Johanne | |
dc.contributor.author | Lavoie, Julie | |
dc.contributor.author | Luo, Hongyu | |
dc.contributor.author | Peng, Junzheng | |
dc.contributor.author | Qi, Shijie | |
dc.contributor.author | Wu, Tao | |
dc.contributor.author | Chen, Fei | |
dc.contributor.author | Shen, Jianzhong | |
dc.contributor.author | Hu, Shenjiang | |
dc.contributor.author | Wu, Jiangping | |
dc.date.accessioned | 2024-06-04T17:30:00Z | |
dc.date.available | NO_RESTRICTION | fr |
dc.date.available | 2024-06-04T17:30:00Z | |
dc.date.issued | 2016-02-05 | |
dc.identifier.uri | http://hdl.handle.net/1866/33322 | |
dc.publisher | American physiological society | fr |
dc.subject | Ephrinb3 | fr |
dc.subject | Estrogen | fr |
dc.subject | Blood pressure | fr |
dc.title | Estrogen and testosterone in concert with EFNB3 regulate vascular smooth muscle cell contractility and blood pressure | fr |
dc.type | Article | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine. École de kinésiologie et des sciences de l'activité physique | fr |
dc.identifier.doi | 10.1152/ajpheart.00873.2015 | |
dcterms.abstract | EPH kinases and their ligands, ephrins (EFNs), have vital and diverse biological functions, although their function in blood pressure (BP) control has not been studied in detail. In the present study, we report that Efnb3 gene knockout (KO) led to increased BP in female but not male mice. Vascular smooth muscle cells (VSMCs) were target cells for EFNB3 function in BP regulation. The deletion of EFNB3 augmented contractility of VSMCs from female but not male KO mice, compared with their wild-type (WT) counterparts. Estrogen augmented VSMC contractility while testosterone reduced it in the absence of EFNB3, although these sex hormones had no effect on the contractility of VSMCs from WT mice. The effect of estrogen on KO VSMC contractility was via a nongenomic pathway involving GPER, while that of testosterone was likely via a genomic pathway, according to VSMC contractility assays and GPER knockdown assays. The sex hormone-dependent contraction phenotypes in KO VSMCs were reflected in BP in vivo. Ovariectomy rendered female KO mice normotensive. At the molecular level, EFNB3 KO in VSMCs resulted in reduced myosin light chain kinase phosphorylation, an event enhancing sensitivity to Ca(2+)flux in VSMCs. Our investigation has revealed previously unknown EFNB3 functions in BP regulation and show that EFNB3 might be a hypertension risk gene in certain individuals. | fr |
dcterms.isPartOf | urn:ISSN:0363-6135 | fr |
dcterms.isPartOf | urn:ISSN:1522-1539 | fr |
dcterms.language | eng | fr |
UdeM.ReferenceFournieParDeposant | doi: 10.1152/ajpheart.00873.2015 | fr |
UdeM.VersionRioxx | Version publiée / Version of Record | fr |
oaire.citationTitle | American journal of physiology. Heart and circulatory physiology | fr |
oaire.citationVolume | 310 | fr |
oaire.citationStartPage | H861 | fr |
oaire.citationEndPage | H872 | fr |
Files in this item
This item appears in the following Collection(s)
This document disseminated on Papyrus is the exclusive property of the copyright holders and is protected by the Copyright Act (R.S.C. 1985, c. C-42). It may be used for fair dealing and non-commercial purposes, for private study or research, criticism and review as provided by law. For any other use, written authorization from the copyright holders is required.