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Now showing items 11-20 of 25
Impaired EAT-4 vesicular glutamate transporter leads to defective nocifensive response of caenorhabditis elegans to noxious heat
(Springer, 2019-04-26)
In mammals, glutamate is an important excitatory neurotransmitter. Glutamate and
glutamate receptors are found in areas specifically involved in pain sensation, transmission
and transduction such as peripheral nervous ...
Actinobacillus pleuropneumoniae induces SJPL cell cycle arrest in G2/M-phase and inhibits porcine reproductive and respiratory syndrome virus replication
(2015-12)
Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens
in the swine industry and causes important economic losses. No effective antiviral drugs against it are ...
Characterization of Endoproteolytic Processing of Dynorphins by Proprotein Convertases using Mouse Spinal Cord S9 Fractions and Mass Spectrometry
(2016-06-01)
Dynorphins are important neuropeptides with a central role in nociception and pain alleviation. Many mechanisms regulate endogenous dynorphin concentrations, including proteolysis. Proprotein convertases (PCs) are widely ...
Tachykinins Processing is Significantly Impaired in PC1 and PC2 Mutant Mouse Spinal Cord S9 Fractions
(2015-11-01)
Substance P (SP) play a central role in nociceptive transmission and it is an agonist of the
Neurokinin-1 receptor located in the lamina I of the spinal cord. SP is a major proteolytic product
of the protachykinin-1 ...
Gastric stability and oral bioavailability of colistin sulfate in pigs challenged or not with Escherichia coli O149: F4 (K88)
(2015-10)
The aim of the present study was to investigate the in vitro gastric stability of colistin sulfate (CS) and its antimicrobial activity against Escherichia coli and to study the impact of ETEC O149: F4 (K88) infection in ...
Assessment of meat authenticity using bioinformatics, targeted peptide biomarkers and high-resolution mass spectrometry
(2015-10-01)
In recent years, we observed a significant increase of food fraud ranging from false label claims to the use of additives and fillers to increase profitability. Recently in 2013, horse and pig DNA were detected in beef ...
Liquid Chromatography-Electrospray Linear Ion Trap Mass Spectrometry Analysis of Targeted Neuropeptides in Tac1-/- Mouse Spinal Cords Reveal Significant Lower Concentration of Opioid Peptides
(2015-08)
Tachykinin and opioid peptides play a central role in pain transmission, modulation and
inhibition. The treatment of pain is very important in medicine and many studies using NK1
receptor antagonists failed to show ...
Assessment of Tandem Mass Spectrometry and High Resolution Mass Spectrometry for the Analysis of Bupivacaine in Plasma
(2015-11-01)
Triple quadrupole mass spectrometers coupled with high performance liquid chromatography are
workhorses in quantitative bioanalyses. It provides substantial benefits including reproducibility,
sensitivity and selectivity ...
In Vitro Ketamine CYP3A Mediated Metabolism Study using Mammalian Liver S9 Fractions, cDNA Expressed Enzymes and Liquid Chromatography Tandem Mass Spectrometry
(2014-12-01)
Ketamine is widely used in medicine in combination with several benzodiazepines including
midazolam. The objectives of this study were to develop a novel HPLC-MS/SRM method
capable of quantifying ketamine and norketamine ...
Evaluation of multiple reaction monitoring cubed for the analysis of tachykinin related peptides in rat spinal cord using a hybrid triple quadrupole–linear ion trap mass spectrometer
(2014-02)
Targeted peptide methods generally use HPLC-MS/MRM approaches. Although dependent on
the instrumental resolution, interferences may occur while performing analysis of complex
biological matrices. HPLC-MS/MRM3 is a ...