Evaluation of multiple reaction monitoring cubed for the analysis of tachykinin related peptides in rat spinal cord using a hybrid triple quadrupole–linear ion trap mass spectrometer
Article [Accepted Manuscript]
Is part of
Journal of chromatography ; vol. 947/948, no. B, Biomedical sciences and applications, pp. 164-167.Abstract(s)
Targeted peptide methods generally use HPLC-MS/MRM approaches. Although dependent on
the instrumental resolution, interferences may occur while performing analysis of complex
biological matrices. HPLC-MS/MRM3 is a technique, which provides a significantly better
selectivity, compared with HPLC-MS/MRM assay. HPLC-MS/MRM3 allows the detection and
quantitation by enriching standard MRM with secondary product ions that are generated within
the linear ion trap. Substance P (SP) and neurokinin A (NKA) are tachykinin peptides playing a
central role in pain transmission. The objective of this study was to verify whether HPLC-HPLCMS/
MRM3 could provide significant advantages over a more traditional HPLC-MS/MRM
assay for the quantification of SP and NKA in rat spinal cord. The results suggest that
reconstructed MRM3 chromatograms display significant improvements with the nearly complete
elimination of interfering peaks but the sensitivity (i.e. signal-to-noise ratio) was severely
reduced. The precision (%CV) observed was between 3.5% - 24.1% using HPLC-MS/MRM
and in the range of 4.3% - 13.1% with HPLC-MS/MRM3, for SP and NKA. The observed
accuracy was within 10% of the theoretical concentrations tested. HPLC-MS/MRM3 may
improve the assay sensitivity to detect difference between samples by reducing significantly the
potential of interferences and therefore reduce instrumental errors.
Other location(s)
Collections
This document disseminated on Papyrus is the exclusive property of the copyright holders and is protected by the Copyright Act (R.S.C. 1985, c. C-42). It may be used for fair dealing and non-commercial purposes, for private study or research, criticism and review as provided by law. For any other use, written authorization from the copyright holders is required.