Differences in platelet-rich plasma composition influence bone healing
Article [Version of Record]
Is part of
Journal of clinical periodontology ; vol. 48, pp. 1613-1623.Publisher(s)
WileyAuthor(s)
Affiliation
Abstract(s)
Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been
used to enhance bone regeneration. Clinical studies, however, reported inconsistent
outcomes. This study aimed to assess the effect of changes in leucocyte and PRP
(L-PRP) composition on bone defect healing.
Materials and Methods: L-PRPs were prepared using different centrifugation
methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP,
double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias
were collected and analysed using micro-CT and histology.
Results: Double-spin L-PRP contained higher concentrations of platelets than singlespin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3
) compared
to single-spin L-PRP (1.42 ± 0.27 mm3
) or filtered L-PRP (1.38 ± 0.28 mm3
). There
were fewer mast cells, lymphocytes, and macrophages in defects treated with
double-spin L-PRP than in those treated with single-spin or filtered L-PRP.
Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.