Quantification of peptides in human synovial fluid using liquid chromatography–tandem mass spectrometry
dc.contributor.author | García Ac, Araceli | |
dc.contributor.author | Duy, Sung Vo | |
dc.contributor.author | Sauvé, Sébastien | |
dc.contributor.author | Moldovan, Florina | |
dc.contributor.author | Roullin, Valérie Gaëlle | |
dc.date.accessioned | 2018-04-25T18:05:16Z | |
dc.date.available | MONTHS_WITHHELD:24 | fr |
dc.date.available | 2018-04-25T18:05:16Z | |
dc.date.issued | 2018-04-06 | |
dc.identifier.uri | http://hdl.handle.net/1866/19943 | |
dc.publisher | Elsevier | fr |
dc.subject | Quantitative bioanalysis | fr |
dc.subject | Tandem mass spectrometry | fr |
dc.subject | Peptide quantification | fr |
dc.subject | B1 receptor | fr |
dc.subject | Endothelin receptor antagonist | fr |
dc.subject | Synovial fluid | fr |
dc.subject | Knee osteoarthritis | fr |
dc.subject | Distribution coefficient | fr |
dc.title | Quantification of peptides in human synovial fluid using liquid chromatography–tandem mass spectrometry | fr |
dc.type | Article | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de pharmacie | fr |
UdeM.statut | Professeur(e) / Professor | fr |
dc.identifier.doi | 10.1016/j.talanta.2018.03.105 | |
dcterms.abstract | A method to explore the stability of two anti-inflammatory peptides in human synovial fluid (HSF) has been developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The two peptides are BQ123 Cyclo(-D-Trp-D-Asp-L-Pro-D-Val-L-Leu, Mw = 610.7) and R-954 (AcOrn[Oic2, (αMe)Phe5, DβNal7, Ile8]desArg9-bradykinin, Mw = 1194.4). Human synovial fluid samples were analyzed after a protein precipitation step with acetonitrile and dilution with mobile phase. DMSO was used as anti-adsorptive agent. We used an octyl silane column with formic acid (0.1%, v/v) in water as the aqueous mobile phase and acetonitrile isopropanol-formic acid (20:80, 0.1 v/v) as the organic mobile phase and 0.7 mL/min flow rate. The peptides CY-771 and pepstatin A were used as internal standards. Selective detection was performed by tandem mass spectrometry with a heated electrospray source (HESI), operated in positive ionization mode and in selected reaction monitoring acquisition (SRM). The method limit of quantification (injection volume = 10 µL) was 0.17 ng and 1.2 ng, corresponding to 28 and 102 nmol L−1 for BQ123 and R-954 respectively in human synovial fluid. Calibration curves obtained using matrix-matched calibration standards and internal standard were linear from 20 to 1000 nmol L−1. Precision values (%R.S.D.) were ≤ 14% in the entire linear range. Accuracy measured at a low and a high concentration level ranged from 93.1% to 102%. The recoveries (at 800 nmol L−1) were 96.4% for BQ123 and 102.0% for R-954. The method was successfully applied to follow the degradation kinetics of both peptides in human synovial fluid from arthritic patients during 72 h. | fr |
dcterms.isPartOf | urn:ISSN:0039-9140 | |
dcterms.isPartOf | urn:ISSN:1873-3573 | |
dcterms.language | eng | fr |
UdeM.VersionRioxx | Version acceptée / Accepted Manuscript | fr |
oaire.citationTitle | Talanta | |
oaire.citationVolume | 186 | |
oaire.citationStartPage | 124 | |
oaire.citationEndPage | 132 |
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