Show item record

dc.contributor.authorDouanne, Noélie
dc.contributor.authorDong, George
dc.contributor.authorAmin, Atia
dc.contributor.authorBernardo, Lorena
dc.contributor.authorBlanchette, Mathieu
dc.contributor.authorLanglais, David
dc.contributor.authorOlivier, Martin
dc.contributor.authorFernandez Prada, Christopher
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.subjectDrug resistancefr
dc.subjectGenetic exchangefr
dc.subjectEukaryotic parasitesfr
dc.subjectCellular cross talkfr
dc.subjectHorizontal gene transferfr
dc.subjectExtracellular vesiclesfr
dc.titleLeishmania parasites exchange drug-resistance genes through extracellular vesiclesfr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine vétérinairefr
dc.contributor.affiliationCentre universitaire de santé McGill.‏ ‎Institut de recherchefr
dc.contributor.affiliationMcGill Genome Centre
dc.contributor.affiliationMcGill University.‏ ‎School of computer science
dc.contributor.affiliationMcGill University Research Centre on Complex Traits
dcterms.abstractLeishmania are eukaryotic parasites that have retained the ability to produce extracellular vesicles (EVs) through evolution. To date, it has been unclear if different DNA entities could be associated with Leishmania EVs and whether these could constitute a mechanism of horizontal gene transfer (HGT). Herein, we investigate the DNA content of EVs derived from drug-resistant parasites, as well as the EVs’ potential to act as shuttles for DNA transfer. Next-generation sequencing and PCR assays confirm the enrichment of amplicons carrying drug-resistance genes associated with EVs. Transfer assays of drug-resistant EVs highlight a significant impact on the phenotype of recipient parasites induced by the expression of the transferred DNA. Recipient parasites display an enhanced growth and better control of oxidative stress. We provide evidence that eukaryotic EVs function as efficient mediators in HGT, thereby facilitating the transmission of drug-resistance genes and increasing the fitness of cells when encountering stressful
UdeM.VersionRioxxVersion publiée / Version of Recordfr
oaire.citationTitleCell reportsfr

Files in this item


This item appears in the following Collection(s)

Show item record

Attribution 4.0 International (CC BY 4.0)
Usage rights : Attribution 4.0 International (CC BY 4.0)