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dc.contributor.authorDenis, Jean-François
dc.contributor.authorSader, Fadi
dc.contributor.authorGatien, Samuel
dc.contributor.authorVilliard, Éric
dc.contributor.authorPhilip, Anie
dc.contributor.authorRoy, Stéphane
dc.date.accessioned2017-11-03T15:39:54Z
dc.date.availableNO_RESTRICTIONfr
dc.date.available2017-11-03T15:39:54Z
dc.date.issued2016-10-01
dc.identifier.urihttp://hdl.handle.net/1866/19573
dc.subjectAxolotlfr
dc.subjectEpimorphicfr
dc.subjectRegenerationfr
dc.subjectTGF-β signalingfr
dc.subjectSmad2fr
dc.subjectSmad3fr
dc.subjectLimbfr
dc.subjectSalamanderfr
dc.subjectUrodelesfr
dc.subjectAmbystoma mexicanumfr
dc.titleActivation of Smad2 but not Smad3 is required to mediate TGF-β signaling during axolotl limb regenerationfr
dc.typeArticlefr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine dentairefr
UdeM.statutProfesseur(e) / Professorfr
dc.identifier.doi10.1242/dev.131466
dcterms.abstractAxolotls are unique among vertebrates in their ability to regenerate tissues, such as limbs, tail and skin. The axolotl limb is the most studied regenerating structure. The process is well characterized morphologically; however, it is not well understood at the molecular level. We demonstrate that TGF-β1 is highly upregulated during regeneration and that TGF-β signaling is necessary for the regenerative process. We show that the basement membrane is not prematurely formed in animals treated with the TGF-β antagonist SB- 431542. More importantly, Smad2 and Smad3 are differentially regulated post-translationally during the preparation phase of limb regeneration. Using specific antagonists for Smad2 and Smad3 we demonstrate that Smad2 is responsible for the action of TGF-β during regeneration, whereas Smad3 is not required. Smad2 target genes (Mmp2 and Mmp9) are inhibited in SB-431542-treated limbs, whereas non-canonical TGF-β targets (e.g. Mmp13) are unaffected. This is the first study to show that Smad2 and Smad3 are differentially regulated during regeneration and places Smad2 at the heart of TGF-β signaling supporting the regenerative process.fr
dcterms.isPartOfurn:ISSN:0950-1991
dcterms.isPartOfurn:ISSN:1477-9129
dcterms.languageengfr
UdeM.VersionRioxxVersion acceptée / Accepted Manuscript
oaire.citationTitleDevelopment
oaire.citationVolume143
oaire.citationIssue19
oaire.citationStartPage3481
oaire.citationEndPage3490


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