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dc.contributor.authorHathroubi, Skander
dc.contributor.authorBeaudry, Francis
dc.contributor.authorProvost, Chantale
dc.contributor.authorMartelet, Léa
dc.contributor.authorSegura, Mariela
dc.contributor.authorGagnon, Carl A.
dc.contributor.authorJacques, Mario
dc.date.accessioned2017-01-18T19:52:20Z
dc.date.availableMONTHS_WITHHELD:12fr
dc.date.available2017-01-18T19:52:20Z
dc.date.issued2016-05-25
dc.identifier.urihttp://hdl.handle.net/1866/16387
dc.subjectActinobacillus pleuropneumoniaefr
dc.subjectBiofilmfr
dc.subjectCytokinesfr
dc.subjectInnate immune cellsfr
dc.subjectLipid Afr
dc.subjectMacrophagesfr
dc.titleImpact of Actinobacillus pleuropneumoniae biofilm mode of growth on the lipid A structures and stimulation of immune cellsfr
dc.typeArticle
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine vétérinairefr
UdeM.statutProfesseur(e) / Professorfr
dc.identifier.doi10.1177/1753425916649676
dcterms.abstractActinobacillus pleuropneumoniae (APP), the etiologic agent of porcine pleuropneumonia, forms biofilms on biotic and abiotic surfaces. APP biofilms confers resistance to antibiotics. To our knowledge, no studies have examined the role of APP biofilm in immune evasion and infection persistence. This study was undertaken to (i) investigate biofilm-associated LPS modifications occurring during the switch to biofilm mode of growth; and (ii) characterize pro-inflammatory cytokines expression in porcine pulmonary alveolar macrophages (PAMs) and proliferation in porcine PBMCs challenged with planktonic or biofilm APP cells. Extracted lipid A samples from biofilm and planktonic cultures were analyzed by HPLC high-resolution, accurate mass spectrometry. Biofilm cells displayed significant changes in lipid A profiles when compared with their planktonic counterparts. Furthermore, in vitro experiments were conducted to examine the inflammatory response of PAMs exposed to UV-inactivated APP grown in biofilm or in suspension. Relative mRNA expression of pro-inflammatory genes IL1, IL6, IL8 and MCP1 decreased in PAMs when exposed to biofilm cells compared to planktonic cells. Additionally, the biofilm state reduced PBMCs proliferation. Taken together, APP biofilm cells show a weaker ability to stimulate innate immune cells, which could be due, in part, to lipid A structure modifications.fr
dcterms.isPartOfurn:ISSN:1753-4267
dcterms.isPartOfurn:ISSN:1753-4259
dcterms.languageengfr
UdeM.VersionRioxxVersion originale de l'auteur / Author's Original
oaire.citationTitleInnate immunity
oaire.citationVolume22
oaire.citationIssue5
oaire.citationStartPage353
oaire.citationEndPage362


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