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dc.contributor.authorBoujenoui, Fatma
dc.contributor.authorNkambeu, Bruno
dc.contributor.authorBen Salem, Jennifer
dc.contributor.authorCastano Uruena, Jesus David
dc.contributor.authorBeaudry, Francis
dc.subjectCannabis sativafr
dc.subjectCaenorhabditis elegansfr
dc.subjectVanilloid receptorfr
dc.titleCannabidiol and tetrahydrocannabinol antinociceptive activity is mediated by distinct receptors in caenorhabditis elegansfr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine vétérinairefr
dcterms.abstractCannabis has gained popularity in recent years as a substitute treatment for pain following the risks of typical treatments uncovered by the opioid crisis. The active ingredients frequently associated with pain-relieving effects are the phytocannabinoids Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD), but their effectiveness and mechanisms of action are still under research. In this study, we used Caenorhabditis elegans, an ideal model organism for the study of nociception that expresses mammal ortholog cannabinoid (NPR-19 and NPR-32) and vanilloid (OSM-9 and OCR-2) receptors. Here, we evaluated the antinociceptive activity of THC and CBD, identifying receptor targets and several metabolic pathways activated following exposure to these molecules. The thermal avoidance index was used to phenotype each tested C. elegans experimental group. The data revealed for the first time that THC and CBD decreases the nocifensive response of C. elegans to noxious heat (32°C – 35°C). The effect was reversed 6h post- CBD exposure but not for THC. Further investigations using specific mutants revealed CBD and THC are targeting different systems, namely the vanilloid and cannabinoid systems, respectively. Proteomic analysis revealed differences following Reactome pathways and Gene Ontology Biological Process database enrichment analyses between CBD or THC-treated nematodes and provided insights into potential targets for future drug
UdeM.ReferenceFournieParDeposantF. Boujenoui, J. Ben Salem, B. Nkambeu, J.D. Castano Uruena and F. Beaudry (2023). Cannabidiol and Tetrahydrocannabinol Antinociceptive Activity is Mediated by Distinct Receptors in Caenorhabditis elegans. Neurochemical Research. Manuscript ID: 5689be3f-bd89-4b89-9f7f-fa3213874e75fr
UdeM.VersionRioxxVersion acceptée / Accepted Manuscriptfr
oaire.citationTitleNeurochemical researchfr

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