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dc.contributor.authorThibodeau, Alexandre
dc.contributor.authorFravalo, Philippe
dc.contributor.authorYergeau, Étienne
dc.contributor.authorArsenault, Julie
dc.contributor.authorLahaye, Ludovic
dc.contributor.authorLetellier, Ann
dc.date.accessioned2015-11-10T17:04:42Z
dc.date.availableNO_RESTRICTIONfr
dc.date.available2015-11-10T17:04:42Z
dc.date.issued2015-07
dc.identifier.citationThibodeau, A., Fravalo, P., Yergeau, É., Arsenault, J., Lahaye, L., & Letellier, A. (2015). Chicken Caecal Microbiome Modifications Induced by Campylobacter jejuni Colonization and by a Non-Antibiotic Feed Additive. PLoS ONE, 10(7), e0131978.fr
dc.identifier.urihttp://hdl.handle.net/1866/12620
dc.titleChicken Caecal Microbiome Modifications Induced by Campylobacter jejuni Colonization and by a Non-Antibiotic Feed Additivefr
dc.typeArticlefr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine vétérinaire. Chaire de recherche en salubrité de viandesfr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine vétérinairefr
UdeM.statutÉtudiant(e) aux cycles supérieurs / Graduate Studentfr
dc.identifier.doi10.1371/journal.pone.0131978
dcterms.abstractCampylobacter jejuni is an important zoonotic foodborne pathogen causing acute gastroenteritis in humans. Chickens are often colonized at very high numbers by C. jejuni, up to 109 CFU per gram of caecal content, with no detrimental effects on their health. Farm control strategies are being developed to lower the C. jejuni contamination of chicken food products in an effort to reduce human campylobacteriosis incidence. It is believed that intestinal microbiome composition may affect gut colonization by such undesirable bacteria but, although the chicken microbiome is being increasingly characterized, information is lacking on the factors affecting its modulation, especially by foodborne pathogens. This study monitored the effects of C. jejuni chicken caecal colonization on the chicken microbiome in healthy chickens. It also evaluated the capacity of a feed additive to affect caecal bacterial populations and to lower C. jejuni colonization. From day-0, chickens received or not a microencapsulated feed additive and were inoculated or not with C. jejuni at 14 days of age. Fresh caecal content was harvested at 35 days of age. The caecal microbiome was characterized by real time quantitative PCR and Ion Torrent sequencing. We observed that the feed additive lowered C. jejuni caecal count by 0.7 log (p<0.05). Alpha-diversity of the caecal microbiome was not affected by C. jejuni colonization or by the feed additive. C. jejuni colonization modified the caecal beta-diversity while the feed additive did not. We observed that C. jejuni colonization was associated with an increase of Bifidobacterium and affected Clostridia and Mollicutes relative abundances. The feed additive was associated with a lower Streptococcus relative abundance. The caecal microbiome remained relatively unchanged despite high C. jejuni colonization. The feed additive was efficient in lowering C. jejuni colonization while not disturbing the caecal microbiome.fr
dcterms.bibliographicCitationPLoS ONE ; vol. 10, no 7
dcterms.description[À l'origine dans / Was originally part of : Fac. Méd. vétérinaire - Chaire de recherche en salubrité des viandes]
dcterms.isPartOfurn:ISSN:1932-6203
dcterms.languageengfr
UdeM.VersionRioxxVersion acceptée / Accepted Manuscript


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