dc.contributor.author | Burgher Pulgaron, Yaima | |
dc.contributor.author | Provost, Chantale | |
dc.contributor.author | Pesant, Marie-Jeanne | |
dc.contributor.author | Gagnon, Carl A. | |
dc.date.accessioned | 2023-09-08T17:59:51Z | |
dc.date.available | NO_RESTRICTION | fr |
dc.date.available | 2023-09-08T17:59:51Z | |
dc.date.issued | 2023-05-20 | |
dc.identifier.uri | http://hdl.handle.net/1866/28644 | |
dc.publisher | MDPI | fr |
dc.rights | Attribution 4.0 International (CC BY 4.0) | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/deed.fr | |
dc.subject | Porcine circovirus | fr |
dc.subject | Swine influenza A virus | fr |
dc.subject | Epithelial cells | fr |
dc.subject | Macrophages | fr |
dc.subject | Co-infection | fr |
dc.subject | Viral pathogenesis | fr |
dc.subject | Virus replication | fr |
dc.title | Porcine circovirus modulates swine influenza virus replication in pig tracheal epithelial cells and porcine alveolar macrophages | fr |
dc.type | Article | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine vétérinaire | fr |
dc.identifier.doi | 10.3390/v15051207 | |
dcterms.abstract | The pathogenesis of porcine circovirus type 2b (PCV2b) and swine influenza A virus
(SwIV) during co-infection in swine respiratory cells is poorly understood. To elucidate the impact
of PCV2b/SwIV co-infection, newborn porcine tracheal epithelial cells (NPTr) and immortalized
porcine alveolar macrophages (iPAM 3D4/21) were co-infected with PCV2b and SwIV (H1N1 or
H3N2 genotype). Viral replication, cell viability and cytokine mRNA expression were determined and
compared between single-infected and co-infected cells. Finally, 30mRNA sequencing was performed
to identify the modulation of gene expression and cellular pathways in co-infected cells. It was found
that PCV2b significantly decreased or improved SwIV replication in co-infected NPTr and iPAM
3D4/21 cells, respectively, compared to single-infected cells. Interestingly, PCV2b/SwIV co-infection
synergistically up-regulated IFN expression in NPTr cells, whereas in iPAM 3D4/21 cells, PCV2b
impaired the SwIV IFN induced response, both correlating with SwIV replication modulation. RNAsequencing analyses revealed that the modulation of gene expression and enriched cellular pathways
during PCV2b/SwIV H1N1 co-infection is regulated in a cell-type-dependent manner. This study
revealed different outcomes of PCV2b/SwIV co-infection in porcine epithelial cells and macrophages
and provides new insights on porcine viral co-infections pathogenesis. | fr |
dcterms.isPartOf | urn:ISSN:1999-4915 | fr |
dcterms.language | eng | fr |
UdeM.ReferenceFournieParDeposant | Burgher Pulgaron Y, Provost C, Pesant MJ, Gagnon CA. Porcine Circovirus Modulates Swine Influenza Virus Replication in Pig Tracheal Epithelial Cells and Porcine Alveolar Macrophages. Viruses. 2023 May 20;15(5):1207. doi: 10.3390/v15051207. PMID: 37243291; PMCID: PMC10222781. | fr |
UdeM.VersionRioxx | Version publiée / Version of Record | fr |
oaire.citationTitle | Viruses | fr |
oaire.citationVolume | 15 | fr |
oaire.citationIssue | 5 | fr |