Antinociceptive activity of vanilloids in caenorhabditis elegans is mediated by the desensitization of the TRPV channel OCR-2 and specific signal transduction pathways
dc.contributor.author | Nkambeu, Bruno | |
dc.contributor.author | Ben Salem, Jennifer | |
dc.contributor.author | Beaudry, Francis | |
dc.date.accessioned | 2023-02-13T12:52:50Z | |
dc.date.available | MONTHS_WITHHELD:12 | fr |
dc.date.available | 2023-02-13T12:52:50Z | |
dc.date.issued | 2023-02-03 | |
dc.identifier.uri | http://hdl.handle.net/1866/27531 | |
dc.publisher | Springer | fr |
dc.subject | Caenorhabditis elegans | fr |
dc.subject | Eugenol | fr |
dc.subject | Capsaicin | fr |
dc.subject | Proteomics | fr |
dc.subject | Mass spectrometry | fr |
dc.subject | Nociception | fr |
dc.subject | Transient receptor potential cation channel | fr |
dc.title | Antinociceptive activity of vanilloids in caenorhabditis elegans is mediated by the desensitization of the TRPV channel OCR-2 and specific signal transduction pathways | fr |
dc.type | Article | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine vétérinaire | fr |
dc.identifier.doi | 10.1007/s11064-023-03876-1 | |
dcterms.abstract | Vanilloids, including capsaicin and eugenol, are ligands of transient receptor potential channel vanilloid subfamily member 1 (TRPV1). Prolonged treatment with vanilloids triggered the desensitization of TRPV1, leading to analgesic or antinociceptive effects. Caenorhabditis elegans (C. elegans) is a model organism expressing vanilloid receptor orthologs (e.g., OSM-9 and OCR-2) that are associated with behavioral and physiological processes, including sensory transduction. We have shown that capsaicin and eugenol hamper the nocifensive response to noxious heat in C. elegans. The objective of this study was to perform proteomics to identify proteins and pathways responsible for the induced phenotype and to identify capsaicin and eugenol targets using a thermal proteome profiling (TPP) strategy. The results indicated hierarchical differences following Reactome Pathway enrichment analyses between capsaicin- and eugenol-treated nematodes. However, both treated groups were associated mainly with signal transduction pathways, energy generation, biosynthesis and structural processes. Wnt signaling, a specific signal transduction pathway, is involved following treatment with both molecules. Wnt signaling pathway is noticeably associated with pain. The TPP results show that capsaicin and eugenol target OCR-2 but not OSM-9. Further protein-protein interaction (PPI) analyses showed other targets associated with enzymatic catalysis and calcium ion binding activity. The resulting data help to better understand the broad-spectrum pharmacological activity of vanilloids. | fr |
dcterms.isPartOf | urn:ISSN:0364-3190 | fr |
dcterms.isPartOf | urn:ISSN:1573-6903 | fr |
dcterms.language | eng | fr |
UdeM.ReferenceFournieParDeposant | Nkambeu B, Salem JB, Beaudry F. Antinociceptive Activity of Vanilloids in Caenorhabditis elegans is Mediated by the Desensitization of the TRPV Channel OCR-2 and Specific Signal Transduction Pathways. Neurochem Res. 2023 Feb 3. doi: 10.1007/s11064-023-03876-1. Epub ahead of print. PMID: 36737562. | fr |
UdeM.VersionRioxx | Version acceptée / Accepted Manuscript | fr |
oaire.citationTitle | Neurochemical research | fr |
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