HCO3 dependent impact of Na+,K+, 2Cl cotransport in vascular smooth muscle excitation-contraction coupling
dc.contributor.author | Koltsova, Svetlana V. | |
dc.contributor.author | Luneva, Oksana G. | |
dc.contributor.author | Lavoie, Julie | |
dc.contributor.author | Tremblay, Johanne | |
dc.contributor.author | Maksimov, Georgy V. | |
dc.contributor.author | Hamet, Pavel | |
dc.contributor.author | Orlov, Serguei | |
dc.date.accessioned | 2022-10-31T15:29:34Z | |
dc.date.available | NO_RESTRICTION | fr |
dc.date.available | 2022-10-31T15:29:34Z | |
dc.date.issued | 2009 | |
dc.identifier.uri | http://hdl.handle.net/1866/27000 | |
dc.publisher | Krager | fr |
dc.subject | Na+ | fr |
dc.subject | K+ | fr |
dc.subject | 2Cl- | fr |
dc.subject | Cotransport | fr |
dc.subject | Vascular smooth muscle cells | fr |
dc.subject | Contraction | fr |
dc.subject | Bicarbonate | fr |
dc.title | HCO3 dependent impact of Na+,K+, 2Cl cotransport in vascular smooth muscle excitation-contraction coupling | fr |
dc.type | Article | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine. Département de médecine | fr |
dcterms.abstract | In smooth muscles, inhibition of Na(+),K(+),2Cl(-) cotransport (NKCC) by bumetanide decreased intracellular Cl(-) content ([Cl(-)](i)) and suppressed the contractions triggered by diverse stimuli. This study examines whether or not bicarbonate, a regulator of several Cl(-) transporters, affects the impact of NKCC in excitation-contraction coupling. Addition of 25 mM NaHCO(3) attenuated the inhibitory action of bumetanide on mesenteric artery contractions evoked by 30 mM KCl and phenylephrine (PE) by 5 and 3-fold, respectively. In cultured vascular smooth muscle cells, NaHCO(3) almost completely abolished inhibitory actions of bumetanide on transient depolarization and [Ca(2+)](i) elevation triggered by PE. In bicarbonate-free medium, bumetanide decreased [Cl(-)](i) by approximately 15%; this effect was almost totally abrogated by NaHCO(3). The addition of NaHCO(3) resulted in 2-fold inhibition of NKCC activity and 3-fold attenuation of [Cl(-)](i). These data strongly suggest that extracellular HCO(3)(-) diminishes the NKCC-sensitive component of excitation-contraction coupling via suppression of this carrier. | fr |
dcterms.isPartOf | urn:ISSN:1015-8987 | fr |
dcterms.isPartOf | urn:ISSN:1421-9778 | fr |
dcterms.language | eng | fr |
UdeM.ReferenceFournieParDeposant | doi: 10.1159/000218187 | fr |
UdeM.VersionRioxx | Version publiée / Version of Record | fr |
oaire.citationTitle | Cellular physiology and biochemistry | fr |
oaire.citationVolume | 23 | fr |
oaire.citationStartPage | 407 | fr |
oaire.citationEndPage | 414 | fr |
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