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dc.contributor.authorHarada, R.
dc.contributor.authorVadnais, C.
dc.contributor.authorSansregret, L.
dc.contributor.authorLeduy, L.
dc.contributor.authorBérubé, G.
dc.contributor.authorRobert, François
dc.contributor.authorNepveu, Alain
dc.date.accessioned2008-04-10T18:55:29Z
dc.date.available2008-04-10T18:55:29Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/1866/2255
dc.description.sponsorshipThe pc3oriPE plasmid and helpful advices were kindly provided by Dr Lori Frappier. A.N. is the recipient of a scholarship from the Fonds de la Recherche en Sante´ du Québec. C.V. is the recipient of a studentship from the McGill University Cancer Consortium Training Grant in Cancer Research (sponsored by CIHR). F.R. holds a new investigator award from the CIHR. This research was supported by grant No. 014288 from the Canadian Cancer Society to A.N. and a grant from Genome Canada/ Génome Québec to F.R and A.N. Funding to pay the Open Access publication charges for this article was provided by grant No. 014288 from the Canadian Cancer Society to A.N.en
dc.format.extent772953 bytes
dc.format.mimetypeapplication/pdf
dc.publisherOxford University Pressen
dc.titleGenome-wide location analysis and expression studies reveal a role for p110 CUX1 in the activation of DNA replication genes
dc.typeArticleen
dc.contributor.affiliationUniversité de Montréal. Faculté de médecinefr
dc.contributor.affiliationUniversité de Montréal. Faculté de médecine. Institut de recherches cliniques de Montréalfr
dcterms.abstractProteolytic processing of the CUX1 transcription factor generates an isoform, p110 that accelerates entry into S phase. To identify targets of p110 CUX1 that are involved in cell cycle progression, we performed genome-wide location analysis using a promoter microarray. Since there are no antibodies that specifically recognize p110, but not the full-length protein, we expressed physiological levels of a p110 isoform with two tags and purified chromatin by tandem affinity purification (ChAP). Conventional ChIP performed on synchronized populations of cells confirmed that p110 CUX1 is recruited to the promoter of cell cycle-related targets preferentially during S phase. Multiple approaches including silencing RNA (siRNA), transient infection with retroviral vectors, constitutive expression and reporter assays demonstrated that most cell cycle targets are activated whereas a few are repressed or not affected by p110 CUX1. Functional classes that were over-represented among targets included DNA replication initiation. Consistent with this finding, constitutive expression of p110 CUX1 led to a premature and more robust induction of replication genes during cell cycle progression, and stimulated the long-term replication of a plasmid bearing the oriP replicator of Epstein Barr virus (EBV).en
dcterms.languageengen
UdeM.VersionRioxxVersion acceptée / Accepted Manuscript
oaire.citationTitleNucleic acids research
oaire.citationVolume36
oaire.citationIssue1
oaire.citationStartPage189
oaire.citationEndPage202


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