dc.contributor.author | Thibodeau, Alexandre | |
dc.contributor.author | Fravalo, Philippe | |
dc.contributor.author | Taboada, Eduardo N. | |
dc.contributor.author | Laurent-Lewandowski, Sylvette | |
dc.contributor.author | Guévremont, Èvelyne | |
dc.contributor.author | Quessy, Sylvain | |
dc.contributor.author | Letellier, Ann | |
dc.date.accessioned | 2015-10-23T16:53:04Z | |
dc.date.available | NO_RESTRICTION | fr |
dc.date.available | 2015-10-23T16:53:04Z | |
dc.date.issued | 2015-05 | |
dc.identifier.uri | http://hdl.handle.net/1866/12388 | |
dc.rights | Ce document est mis à disposition selon les termes de la Licence Creative Commons Paternité 4.0 International. / This work is licensed under a Creative Commons Attribution 4.0 International License. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Campylobacter jejuni | fr |
dc.subject | Chicken | fr |
dc.subject | Colonization | fr |
dc.subject | CGF typing | fr |
dc.subject | Autoagglutination | fr |
dc.subject | Chemotaxis | fr |
dc.subject | Adhesion and invasion | fr |
dc.subject | Competition | fr |
dc.title | Extensive characterization of Campylobacter jejuni chicken isolates to uncover genes involved in the ability to compete for gut colonization | fr |
dc.type | Article | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine vétérinaire. Chaire de recherche en salubrité de viandes | fr |
dc.contributor.affiliation | Université de Montréal. Faculté de médecine vétérinaire | fr |
UdeM.statut | Étudiant(e) aux cycles supérieurs / Graduate Student | fr |
dc.identifier.doi | 10.1186/s12866-015-0433-5 | |
dcterms.abstract | Background: Campylobacter jejuni is responsible for human foodborne enteritis. This bacterium is a remarkable
colonizer of the chicken gut, with some strains outcompeting others for colonization. To better understand this
phenomenon, the objective of this study was to extensively characterize the phenotypic performance of C. jejuni
chicken strains and associate their gut colonizing ability with specific genes. Results: C. jejuni isolates (n = 45) previously analyzed for the presence of chicken colonization associated genes
were further characterized for phenotypic properties influencing colonization: autoagglutination and chemotaxis
as well as adhesion to and invasion of primary chicken caecal cells. This allowed strains to be ranked according to
their in vitro performance. After their in vitro capacity to outcompete was demonstrated in vivo, strains were then
typed by comparative genomic fingerprinting (CGF). In vitro phenotypical properties displayed a linear variability
among the tested strains. Strains possessing higher scores for phenotypical properties were able to outcompete
others during chicken colonization trials. When the gene content of strains was compared, some were associated
with different phenotypical scores and thus with different outcompeting capacities. Use of CGF profiles showed
an extensive genetic variability among the studied strains and suggested that the outcompeting capacity is not
predictable by CGF profile. Conclusion: This study revealed a wide array of phenotypes present in C. jejuni strains, even though they were
all recovered from chicken caecum. Each strain was classified according to its in vitro competitive potential and its
capacity to compete for chicken gut colonization was associated with specific genes. This study also exposed the
disparity existing between genetic typing and phenotypical behavior of C. jejuni strains. | fr |
dcterms.description | [À l'origine dans / Was originally part of : Fac. Méd. vétérinaire - Chaire de recherche en salubrité des viandes] | |
dcterms.isPartOf | urn:ISSN:1471-2180 | |
dcterms.language | eng | fr |
UdeM.VersionRioxx | Version acceptée / Accepted Manuscript | |
oaire.citationTitle | BMC microbiology | |
oaire.citationVolume | 15 | |